Instrumental Sample Analyses W912HZ25Q0021
Summary
AI-generated · Aug 24, 2025Contractor will perform instrumental sample analyses across multiple areas: molecular signaling by measuring peptide/protein levels using HPLC and LC-MS to identify the best method to produce optimal signaling within a cyanobacterial colony and to support model development; phage particle measurement and characterization using TEM/SEM to describe phage isolates; cyanobacteria inhibition by assessing peptide/protein levels via HPLC/LC-MS to evaluate the effectiveness of plasmids carrying genes that alter circadian rhythm; and trehalose measurement by HPLC and/or LC-MS to determine effects on cell survival during desiccation.
Deliverables include monthly progress reports, the methods used, and the data/results. The data will help determine optimal signaling within the colony and the best parameters to support model development. This notice expands from a generic scope to a detailed, technique-specific set of analyses (HPLC/LC-MS for signaling and trehalose, TEM/SEM for phage), outlining the expected data products and cadence.
Objectives: The contractor shall perform molecular signaling analyses using peptide/protein measurement via HPLC, LC-MS analyses which will allow researchers to determine the best method to produce optimal signaling within a colony and will support model development; phage particle measurement using Transmission Electron Microscopy/Scanning Electron Microscopy (TEM/SEM) analyses which will be used to describe and characterize phage isolates; cyanobacteria inhibition using peptide/protein measurement using via HPLC, LC-MS analyses to determine effectiveness of plasmids containing genes to alter circadian rhythm of cyanobacteria; and trehalose measurement via High Performance Liquid Chromatography (HPLC) and/or Liquid Chromatography-Mass Spectrometry (LC-MS) analyses to determine the impact on cell survival during desiccation. Deliverables include monthly progress reports, methods used, and results (data). The data obtained from these analyses will allow researchers to determine the best method to produce optimal signaling within a colony and determine optimal parameters to support model development.
From Solicitation posted on Jun 10, 2025Objectives: The contractor shall perform molecular signaling analyses using peptide/protein measurement via HPLC, LC-MS analyses which will allow researchers to determine the best method to produce optimal signaling within a colony and will support model development; phage particle measurement using Transmission Electron Microscopy/Scanning Electron Microscopy (TEM/SEM) analyses which will be used to describe and characterize phage isolates; cyanobacteria inhibition using peptide/protein measurement using via HPLC, LC-MS analyses to determine effectiveness of plasmids containing genes to alter circadian rhythm of cyanobacteria; and trehalose measurement via High Performance Liquid Chromatography (HPLC) and/or Liquid Chromatography-Mass Spectrometry (LC-MS) analyses to determine the impact on cell survival during desiccation. Deliverables include monthly progress reports, methods used, and results (data). The data obtained from these analyses will allow researchers to determine the best method to produce optimal signaling within a colony and determine optimal parameters to support model development.
From Solicitation posted on Jul 17, 2025Notice history
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Solicitation LATEST Posted Jul 17, 2025View changes (2)
- Description: Description was updated
- Response Deadline: Jun 16, 2025 → Aug 18, 2025
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USA